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The production of itaconic acid by Aspergillus terreus NRRL 1960 was studied. The optimal culture conditions, such as pH, inoculum size and medium composition were established. Maximum production of itaconic acid, 19.18g/~, was obtained when the cultivation was carried out at 371C and pH 2.5 for 7days, with medium containing 5 % (w/v) glucose, 0.5% (w/v) NH4C1, 0.2% (w/v) yeast extract, 0.1 % (w/v) CaCIZ, 0.1 % ( w/v) MgSO, and 0.2 % ( w/v) NaCI. A proper medium for inoculum culture was found to be 2%(w/v) malt extract. The batch production of itaconic acid with free cells in a stirrdtank reactor was not efficient, compared to the shake-flask culture.
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